Studying mitochondrial function and glycolytic flux is crucial for the understanding of cell biology, in particular in the fields of pharmacology, toxicology, oncology, biochemistry and nutrition. Such investigations can be carried out using a Seahorse Xfe24 (Agilent) apparatus, which has been financed by the CPER within the framework of the GIS Nutrition, Food, Metabolism and Health (NAMS). This apparatus enables the measurement of oxygen consumption in order to evaluate mitochondrial function using different types of substrates and metabolic modulators. The glycolytic flux within the cells can also be assessed through the acidification of the extra-cellular culture medium. Regarding mitochondrial function, the Seahorse technology allows the measurement of different respiratory parameters such as basal respiration or maximum respiration after the addition of FCCP, a powerful uncoupler of oxidative phosphorylation. This technology can be used to study metabolic function on adherent cells, 3D cell systems such as organoids and also on zebrafish embryos.
Aude Bodin (email@example.com ; NuMeCan)
Bernard Fromenty (NuMeCan),
Dominique Lagadic-Gossmann (Irset)